Question In slave mode, while reading data from “ftdi_slave_read” it is giving a data frame which is not transmitted from the opposite end. Below data is transmitted from the opposite end(line 7) Analyzer screenshot where data shows mismatching Data received from LabVIEW, notice line no 7 where data is mismatching. I feel that it taking from the FTDI chip buffer. Is there any steps or function to clear buffer data of FTDI hardware? Answer Scope/analyzer shows an extra nibble (one byte + a nibble) in every frame. The driver's text file report shows only the first byte and doesn't show the extra nibble of the frame. There could be a setting in the analyzer/scope which shows the extra nibble that confuses you.